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Updated: Oct 8, 2025
An Optimized Hemagglutination Inhibition HI Assay to Quantify Influenza-specific Antibody Titers
Published on: December 1, 2017
Accuracy assessment of total or IgG Immunoglobulin to hepatitis A virus tests around immunity threshold
Lina Mouna1, Sepideh Akhavan2, Abir Jadoui3
1Universite Paris Saclay, INSERM U1193, AP-HP, Hôpital Paul Brousse, Virology & National Reference Center for HAV, Villejuif, 94800 France.
Background:
Anti-hepatitis A virus (HAV) antibody titers at 20 IU/L are assumed to correlate with protection against HAV challenge.
Methods:
We examined the accuracy and precision of currently in use immunoassays for total or anti-HAV IgG determination, by repeated testing of dilutions of the international anti-HAV standard, within a 10-50 IU/mL concentration range.
Results And Conclusion:
Eight immunoassays were evaluated. All could confidently identify people who need to be vaccinated, or who might benefit from a booster vaccine: no positive interpretation for the 10 and 15 IU/mL concentrations. However, qualitative interpretation may differ from test to test in the 15-30 IU/mL range. This variation has to be taken into account when comparing seroprevalence data.
