Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intramolecular charge transfer endows near-infrared mitochondrial targeted photosensitizers with enhanced photodynamic therapy.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Size switchable nanomodulator achieving ratio-precise dual-drug codelivery for synergistic glutamine metabolism modulation in pancreatic cancer.

Biomaterials·2026
Same author

Online antibiotic purchase in mainland China: A cross-sectional Simulated Patient study.

Public health·2026
Same author

Single-nucleus RNA sequencing reveals a spatiotemporal pattern of H<sub>2</sub>S signaling in Chinese cabbage.

Science China. Life sciences·2026
Same author

Curcumin and its nano formulation inhibit proliferation and FGF21 expression in esophageal squamous cell carcinoma.

Biochemical and biophysical research communications·2026
Same author

Red Emissive Carbon Dots Photosensitizers for Plant Protection: Highly Effective and Plant-Safe Photodynamic Inactivation of <i>Botrytis cinerea</i>.

Journal of agricultural and food chemistry·2026

Related Experiment Video

Updated: Oct 8, 2025

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
10:44

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Published on: October 21, 2016

30.9K

Profiling Cystathionine β/γ-Lyase in Complex Biosamples Using Novel Activatable Fluorogens.

Yan Jia1, Yiying Wang2, Yongli Zhang3

  • 1State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, China.

Analytical Chemistry
|December 27, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed novel fluorescent probes to directly measure cystathionine lyase (CBL) activity in biological samples. These probes offer a reliable method for studying the enzyme

More Related Videos

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
10:44

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Published on: March 28, 2017

10.0K
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
10:50

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Published on: March 14, 2019

8.3K

Related Experiment Videos

Last Updated: Oct 8, 2025

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
10:44

Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions

Published on: October 21, 2016

30.9K
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
10:44

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Published on: March 28, 2017

10.0K
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
10:50

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Published on: March 14, 2019

8.3K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Cystathionine lyase is crucial in transsulfuration pathways, impacting numerous physiological and pathophysiological processes.
  • Current methods for monitoring cystathionine lyase activity in biological samples are limited, leading to unreliable results.
  • The hydrogen sulfide/cystathionine lyase system's role in disease requires precise molecular monitoring tools.

Purpose of the Study:

  • To design and develop novel activatable fluorescent probes for direct monitoring of cystathionine lyase activity.
  • To create probes based on the naphthylamide scaffold with specific recognition moieties and self-immolative linkers.
  • To establish reliable methods for quantifying cystathionine lyase (CBL and CSE) levels in complex biological systems.

Main Methods:

  • Development of naphthylamide-based fluorescent probes (CBLP and CSEP) incorporating cysteine/homocysteine recognition and a carbamate ethyl sulfide linker.
  • In vitro assessment of probe selectivity and sensitivity for cystathionine beta-lyase (CBL) and cystathionine gamma-lyase (CSE).
  • Application of probes in various biological models, including plant roots, cell lines, zebrafish, and septic rat liver tissue.

Main Results:

  • CBLP demonstrated high selectivity and sensitivity for semiquantifying CBL levels in Arabidopsis thaliana roots and mutants.
  • CSEP successfully detected CSE levels in HCC-LM3 cells, zebrafish, and upregulated CSE in septic rat liver tissue.
  • Probe performance was validated using Western blotting and immunohistochemical analysis, confirming their efficacy.

Conclusions:

  • The developed fluorescent probes provide an effective strategy for reporting cystathionine lyase activity.
  • This approach facilitates the profiling of cystathionine lyase in diverse biological processes.
  • The study paves the way for accurate and efficient direct estimation of cystathionine lyase activity in biological research and diagnostics.