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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

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Self-Assembled Peptide-Labeled Probes for Agglutination-Based Sensing.

Anura Shrestha¹, Butaek Lim¹, Priyanka Shiveshwarkar¹

¹Department of Bioengineering, University of Texas at Arlington, Arlington, Texas, 76010, U.S.A.

Macromolecular Research

|December 27, 2021

Summary

Researchers developed a new peptide-based polydiacetylene (PDA) vesicle probe for rapid agglutination sensing. This novel biosensor effectively detects multivalent targets like streptavidin, showing promise for future diagnostic applications.

Keywords:

agglutination amphiphile detection peptide polydiacetylene

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Area of Science:

Biomaterials Science
Nanotechnology
Analytical Chemistry

Background:

Polydiacetylene (PDA) vesicles are stimuli-responsive materials with useful optical properties for sensing.
Agglutination-based sensing using PDA vesicles is an underutilized but promising technique.
Developing rapid and sensitive detection methods for multivalent targets is crucial in diagnostics.

Purpose of the Study:

To create a novel agglutination probe using peptide-displaying polydiacetylene vesicles.
To demonstrate proof-of-concept detection of a multivalent target, streptavidin, using this probe.
To investigate the relationship between vesicle composition and detection performance.

Main Methods:

Functionalization of diacetylene amphiphiles with a biotin mimetic peptide.
Formation of peptide-displaying polydiacetylene vesicles.
Optimization of vesicle composition to determine optimal peptide surface density.
Testing the agglutination response to the multivalent target streptavidin.

Main Results:

Successful generation of a peptide-functionalized polydiacetylene vesicle probe.
Demonstrated proof-of-concept detection of streptavidin via an effective agglutination response.
Identified a limit for peptide amphiphile surface density on the vesicles.
Achieved a wide operational detection range for the target.

Conclusions:

Peptide-displaying polydiacetylene vesicles can be rapidly generated for agglutination-based sensing.
The developed probe shows effective detection of streptavidin, highlighting its potential for multivalent target analysis.
Future formulations may be applicable for profiling a broader range of multivalent targets.