Related Experiment Video

Updated: Aug 15, 2026

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
14:58

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Published on: November 12, 2012

Two chromatographically separable forms of Escherichia coli elongation factor Tu

M Geiser, J Gordon

    Proceedings of the National Academy of Sciences of the United States of America
    |March 1, 1978
    PubMed

    More Related Videos

    Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function08:03

    Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA Function

    Published on: June 21, 2017

    Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli10:34

    Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

    Published on: August 22, 2017

    Related Concept Videos

    Improving Translational Accuracy02:07

    Improving Translational Accuracy

    Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
    Tail-anchoring of Proteins in the ER Membrane01:45

    Tail-anchoring of Proteins in the ER Membrane

    Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
    Stringent Response in E. coli01:23

    Stringent Response in E. coli

    Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...