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IgM binding protein expressed by activated B cells.

S K Sanders, H Kubagawa, T Suzuki

    Journal of Immunology (Baltimore, Md. : 1950)
    |July 1, 1987
    PubMed
    Summary
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    Researchers discovered a new IgM binding protein on activated B lymphocytes. This 60-kDa protein also appears on chronic lymphocytic leukemia cells and binds both human and mouse IgM.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • B lymphocytes play a crucial role in the adaptive immune response.
    • Immunoglobulin M (IgM) is a key antibody involved in the early stages of humoral immunity.

    Purpose of the Study:

    • To identify and characterize a novel protein expressed on activated B lymphocytes.
    • To investigate the binding capabilities of this protein with IgM molecules.

    Main Methods:

    • Phorbol myristate acetate (PMA) was used to activate B lymphocytes.
    • Immunofluorescence analysis and Western blotting were employed to detect and analyze the protein.
    • The binding of IgM to the protein was assessed using secreted and cross-linked membrane-bound IgM.

    Main Results:

    • A 60-kDa IgM binding protein was identified on the surface of B lymphocytes within 18 hours of PMA activation.

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  • This protein was also detected on fresh leukemic B cells from chronic lymphocytic leukemia patients, with increased expression after PMA activation.
  • The 60-kDa protein bound both secreted and cross-linked membrane-bound IgM from human and mouse sources.
  • Resting and activated T cells, monocytes, and polymorphonuclear leukocytes did not express detectable levels of this protein.

    • Conclusions:

    • A novel 60-kDa IgM binding protein is specifically expressed on activated human B lymphocytes and certain B cell leukemias.
    • This protein facilitates the binding of IgM to the surface of activated B cells, suggesting a role in B cell function or immune regulation.