Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

iPS Cell Differentiation01:22

iPS Cell Differentiation

2.8K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Virus-specific T-cell therapy for neuroinvasive West Nile virus.

The Lancet. Neurology·2026
Same author

Photon-counting CT for dynamic lung perfusion: validation of a low-dose protocol in a porcine lung transplantation model.

European radiology experimental·2026
Same author

Developing and Assessing the Performance of a Machine Learning Model for Analyzing Drinking Behaviors in Minipigs for Experimental Research.

Sensors (Basel, Switzerland)·2026
Same author

Improved Antitumor Activity of Interleukin-12-Secreting Chimeric Antigen Receptor T Cells Targeting CD176 across Different Carcinomas.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie·2026
Same author

Vitamin C Conditioning Generates Tumor-Targeting CAR T Cells with Superior Cytotoxicity and Fitness in a Posttransplant Lymphoproliferative Disorder Tumor Model.

Molecular cancer therapeutics·2025
Same author

Multimodal Imaging of the Corneal Endothelial Transition Zone Reveals Progenitor Cell Population.

Cells·2025
Same journal

Research advances and application prospects of CAR-T therapy in the treatment of age-related diseases.

Frontiers in immunology·2026
Same journal

Machine learning-driven identification and immunohistochemical validation of an integrated immune-inflammatory phenotype for disease-free survival stratification in breast cancer.

Frontiers in immunology·2026
Same journal

Modified treatment protocol for pediatric systemic lupus erythematosus-associated hemophagocytic lymphohistiocytosis with central nervous system involvement: a case report.

Frontiers in immunology·2026
Same journal

Exploratory characterization of IgG1/IgG4 glycosylation and monocyte-derived dendritic cell responses in esophageal squamous cell carcinoma.

Frontiers in immunology·2026
Same journal

JAK-STAT pathway-associated skin diseases: a refined functional framework for inflammatory skin diseases.

Frontiers in immunology·2026
Same journal

Cross-talk among novel programmed cell death pathways: a decisive network in renal ischemia-reperfusion injury.

Frontiers in immunology·2026
See all related articles

Related Experiment Video

Updated: Oct 8, 2025

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research
10:11

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Published on: October 27, 2023

1.2K

Genetic Modification of Limbal Stem Cells to Decrease Allogeneic Immune Responses.

Emilio Valdivia1, Marina Bertolin2, Claudia Breda2

  • 1Institute of Transfusion Medicine and Transplant Engineering, Hannover, Germany.

Frontiers in Immunology
|December 27, 2021
PubMed
Summary
This summary is machine-generated.

Genetic engineering silenced human leukocyte antigen (HLA) expression in limbal stem cells (LSCs), reducing immune rejection after transplantation. This strategy enhances graft survival for limbal stem cell deficiency (LSCD) patients.

Keywords:
HLARNA interferenceallotransplantationgene therapylentiviral vectorlimbal stem celllimbal stem cell deficiency

More Related Videos

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse
04:55

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse

Published on: November 17, 2016

8.5K
Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
11:13

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

9.3K

Related Experiment Videos

Last Updated: Oct 8, 2025

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research
10:11

Author Spotlight: Standardizing Limbal Niche Cell (LNC) Isolation and Characterization to Support Widespread LNC Research

Published on: October 27, 2023

1.2K
A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse
04:55

A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse

Published on: November 17, 2016

8.5K
Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
11:13

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

9.3K

Area of Science:

  • Ophthalmology
  • Immunology
  • Regenerative Medicine

Background:

  • Limbal stem cell (LSC) transplantation is crucial for treating LSC deficiency (LSCD).
  • Allogeneic LSC transplantation faces challenges due to human leukocyte antigen (HLA) variability, impacting graft survival.
  • Understanding LSC immunogenicity is key to improving transplantation outcomes.

Purpose of the Study:

  • To characterize the immunologic properties of LSCs.
  • To develop a genetic engineering strategy to reduce LSC immunogenicity for enhanced allogeneic graft survival.
  • To assess the impact of HLA silencing on LSC function and immune response.

Main Methods:

  • LSC HLA class I and II expression was silenced using lentiviral vectors encoding short hairpin RNAs targeting β2M and CIITA.
  • LSCs were analyzed for HLA expression under inflammatory conditions.
  • T-cell activation, proliferation, and cytotoxicity assays were performed.
  • Antibody-mediated cellular-dependent cytotoxicity was assessed.

Main Results:

  • LSCs constitutively express HLA class I and upregulate HLA class II under inflammation.
  • LSCs induce T-cell mediated immune responses.
  • HLA-silenced LSCs prevented T-cell activation, proliferation, and cytotoxicity.
  • HLA-silenced LSCs were protected from antibody-mediated cellular-dependent cytotoxicity.
  • Genetic modification did not alter LSC phenotypical or functional characteristics.

Conclusions:

  • Genetic engineering can successfully reduce LSC immunogenicity by silencing HLA expression.
  • Low-immunogenic LSCs maintain their typical features and function.
  • This approach offers a proof-of-concept for generating LSCs that support long-term allogeneic graft survival.