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MiR-103a-3p aggravates renal cell carcinoma by targeting TMEM33

Jingyu Zhang1, Qingbo Lu2, Haigang Pang3

  • 1Department of Urology, The Fourth People's Hospital of Shenyang Shenyang 110031, Liaoning Province, China.

American Journal of Translational Research
|December 27, 2021
PubMed
Abstract

Insights

MicroRNA-103a-3p promotes renal cell carcinoma (RCC) progression by targeting TMEM33. This finding offers potential new biomarkers and therapeutic strategies for RCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Renal cell carcinoma (RCC) is a significant global health concern.
  • Understanding the molecular mechanisms driving RCC progression is crucial for developing effective treatments.

Purpose of the Study:

  • To elucidate the role of microRNA-103a-3p (miR-103a-3p) in renal cell carcinoma (RCC) progression.
  • To identify the downstream targets and regulatory pathways involved in miR-103a-3p-mediated RCC development.

Main Methods:

  • Quantitative analysis of miR-103a-3p expression in clinical RCC samples and cell lines.
  • In vitro manipulation of miR-103a-3p levels (inhibition/overexpression) in RCC cell lines (786-O and UO31).
  • Bioinformatics analysis, dual-luciferase reporter assays, and rescue experiments to investigate the interaction between miR-103a-3p and TMEM33.

Main Results:

  • miR-103a-3p expression levels were found to be closely associated with RCC development.
  • A direct interaction between TMEM33 and miR-103a-3p was confirmed through bioinformatics and experimental assays.
  • Overexpression of TMEM33 effectively counteracted the pro-cancer effects of miR-103a-3p in RCC cells.

Conclusions:

  • miR-103a-3p functions as an oncomiR in RCC, promoting cancer progression by regulating TMEM33.
  • The miR-103a-3p/TMEM33 axis represents a potential target for novel prognostic markers and therapeutic interventions in RCC.
Keywords:
Renal cell carcinomaTMEM33metastasismiR-103a-3pproliferation

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