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Related Experiment Video

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High-Sensitive Surface Plasmon Resonance Imaging Biosensor Based on Dual-Wavelength Differential Method.

Youjun Zeng1, Jie Zhou1, Wei Sang1

  • 1Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, Hong Kong SAR, China.

Frontiers in Chemistry
|December 27, 2021
PubMed
Summary

A novel intensity interrogation surface plasmon resonance imaging technique enhances biomolecule detection sensitivity. This improved method offers faster, more accurate real-time monitoring for high-throughput applications.

Keywords:
biomolecule interactionbiophotonics and plasmonicsbiosensing and bioimagingsurface plasmonsurface plasmon sensors

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Area of Science:

  • Biophotonics
  • Surface Plasmon Resonance (SPR) Technology
  • Biosensing

Background:

  • Intensity interrogation surface plasmon resonance (ISPR) is widely used but has limited sensitivity, particularly in imaging (ISPRi).
  • Existing ISPRi methods struggle with precise, high-throughput biomolecular binding detection.

Purpose of the Study:

  • To develop a novel ISPRi technique with enhanced sensitivity for real-time biomolecule detection.
  • To optimize dual-wavelength parameters for maximizing sensitivity in ISPRi.

Main Methods:

  • Utilizing the differential value of two intensities at specific wavelengths from the reflected light spectrum for ISPRi.
  • Optimizing dual-wavelength selection for varied experimental conditions.
  • Real-time monitoring of biomolecular binding events.

Main Results:

  • Achieved a refractive index resolution (RIR) of 2.24 × 10-6 RIU, significantly surpassing traditional ISPRi.
  • Demonstrated real-time, high-throughput detection of biomolecular binding.
  • Validated the effectiveness of the optimized dual-wavelength approach.

Conclusions:

  • The new dual-wavelength ISPRi technique offers substantially improved sensitivity and resolution.
  • This advancement enables faster and more accurate real-time monitoring of biomolecular interactions.
  • The developed method is poised to advance the field of SPRi technologies for various applications.