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miR-149 Suppresses the Proliferation and Metastasis of Human Gastric Cancer Cells by Targeting FOXC1

Dan Li1,2, Yunqing Zhang3, Yulong Li4

  • 1Department of Cell Biology and Genetics, Medical College of Yan'an University, Yan'an, 716000 Shaanxi Province, China.

Biomed Research International
|December 27, 2021
PubMed
Abstract

Insights

MicroRNA-149 (miRNA-149) suppresses gastric cancer progression by targeting FOXC1. Restoring miRNA-149 levels offers a potential therapeutic strategy for gastric cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Gastric cancer is a prevalent malignancy worldwide.
  • MicroRNAs (miRNAs) are key regulators of gene expression, impacting various cellular processes.
  • Dysregulation of miRNAs is implicated in cancer development and progression.

Purpose of the Study:

  • To investigate the functional role of miRNA-149 and FOXC1 in gastric cancer.
  • To elucidate the regulatory relationship between miRNA-149 and FOXC1 in gastric cancer cells.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for expression analysis.
  • Cell culture, transfection with miRNA-149 overexpression plasmid or FOXC1 siRNA.
  • Functional assays including MTT, colony formation, flow cytometry, wound healing, and Transwell assays.
  • Dual-luciferase reporter assay and Western blotting to confirm target interaction.

Main Results:

  • miRNA-149 was significantly underexpressed in gastric cancer tissues and cell lines.
  • Overexpression of miRNA-149 induced apoptosis, cell cycle arrest, and inhibited proliferation and migration.
  • miRNA-149 directly targets FOXC1, which was highly expressed in gastric cancer.
  • Silencing FOXC1 mimicked the inhibitory effects of miRNA-149 on gastric cancer cell behavior.

Conclusions:

  • miRNA-149 acts as a tumor suppressor in gastric cancer by targeting FOXC1.
  • The miRNA-149/FOXC1 axis represents a potential therapeutic target for human gastric cancer.

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