Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dysregulation of Neutrophil-Endothelial Communication in Sepsis: Mechanisms and Therapeutic Perspectives.

Cells·2026
Same author

Enhancing glioma therapy via intranasal administration of F<sub>O</sub>F<sub>1</sub>-ATPase motor-embedded chromatophore nanorockets.

International journal of pharmaceutics·2026
Same author

Two-birds-one-stone, microfluidic producing DES/W microemulsions to solubilize quercetin and penetrate intestinal mucosa for enhanced oral bioavailability.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2026
Same author

Chitosan Nanoparticles for Pulmonary Delivery of Curcumin/Nintedanib to Treat Pulmonary Fibrosis.

International journal of nanomedicine·2025
Same author

Retraction notice to "Photopolymerizable, immunomodulatory hydrogels of gelatin methacryloyl and carboxymethyl chitosan as all-in-one strategic dressing for wound healing" [Int. J. Biol. Macromol. 253 (2023) 127151].

International journal of biological macromolecules·2025
Same author

Prioritizing FDA approved therapeutics for treating sepsis phenotypes: A network modeling approach based on neutrophil proteomics.

Frontiers in immunology·2025

Related Experiment Video

Updated: Oct 8, 2025

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
12:55

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Published on: December 9, 2021

3.4K

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation.

Qingliang Yang1, Jordan C Langston2, Yuan Tang3

  • 1Department of Mechanical Engineering, Temple University.

Journal of Visualized Experiments : Jove
|December 27, 2021
PubMed
Summary

A novel biomimetic microfluidic assay (bMFA) accurately models leukocyte-endothelial cell interactions during inflammation. This tool aids in understanding inflammatory diseases and screening potential therapeutics for better clinical translation.

More Related Videos

Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
09:01

Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

Published on: December 30, 2014

9.8K
Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
05:12

Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice

Published on: August 13, 2015

12.7K

Related Experiment Videos

Last Updated: Oct 8, 2025

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
12:55

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Published on: December 9, 2021

3.4K
Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
09:01

Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

Published on: December 30, 2014

9.8K
Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
05:12

Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice

Published on: August 13, 2015

12.7K

Area of Science:

  • Biomedical Engineering
  • Immunology
  • Microfluidics

Background:

  • Leukocyte-endothelial cell interactions are crucial in inflammatory diseases like sepsis.
  • Excessive leukocyte migration into organs can cause organ failure during inflammation.

Purpose of the Study:

  • To develop and validate a biomimetic microfluidic assay (bMFA) for studying leukocyte-endothelial cell interactions.
  • To investigate the impact of shear rate and vascular topology on these interactions.
  • To provide a platform for rapid therapeutic screening.

Main Methods:

  • Digitized in vivo rodent microvascular networks using GIS and microfabricated PDMS devices.
  • Employed Computational Fluid Dynamics (CFD) to map shear rates and velocities.
  • Validated the bMFA using experimental and computational techniques.

Main Results:

  • The bMFA successfully replicates the leukocyte rolling, adhesion, and migration cascade.
  • Quantified key parameters like rolling velocity, adhesion, migration, and endothelial permeability.
  • Demonstrated the assay's utility with human cells for therapeutic screening.

Conclusions:

  • The bMFA is a physiologically relevant tool for studying leukocyte-endothelial cell interactions in inflammatory conditions.
  • It offers a platform for evaluating therapeutic interventions with improved clinical translatability.