The pH dependence of emulsifying properties for glutathione disulfide at oil-water interfaces

Although peptides were widely used in many fields, their interface behaviors as surfactants have not been explored. The results of the surface tension experiments by an automatic surface tension meter indicate that the stability and emulsifying ability of glutathione disulfide (GSSG) under alkaline conditions were stronger than those under acidic conditions. With encoding the different oxygen and nitrogen atoms in GSSG, as well as the different hydrogen atoms bonded with oxygen and nitrogen atoms. The pH Dependence of the number of hydrogen bonds, affected by the protonation and deprotonation of the functional groups in GSSG, is calculated by LAMMPS software. The results demonstrate that GSSG forms twice as many hydrogen bonds under alkaline conditions as under acidic conditions, resulting in a better surface-interface activity in alkaline conditions. The interface properties of the GSSG surfactant can be regulated by pH. Therefore, GSSG is a potential pH-responsive surfactant.