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Development of a signal-enhanced LFIA based on tyramine-induced AuNPs aggregation for sensitive detection of
Huanhuan Bao1, Meifang Yuan2, Chengui Xiao3
1State Key Laboratory of Food Science and Technology, Nanchang University 235, East Nanjing Road, Nanchang 330047, China.
Abstract:
A signal-enhanced LFIA based on tyramine (TYR)-induced AuNPs aggregation has been developed for the sensitive detection of danofloxacin (DAN). In the model, the hydroxyl radical produced by HRP catalyzing H2O2 can trigger the TYR-AuNPs to aggregate on the T or C line for enhancing the detection signal. The linear range of TYR-AuNPs LFIA was 0.25-5 ng mL-1 with the limit of detection (LOD) of 0.032 ng mL-1, and the LOD was 8-fold lower than that of the traditional AuNPs LFIA (0.26 ng mL-1). The TYR-AuNPs LFIA could be used with the naked eyes to qualitatively detect DAN with a cut-off limit of 2.5 ng mL-1, which was 4-fold lower than that of the traditional AuNPs LFIA (10 ng mL-1). The recoveries of TYR-AuNPs LFIA were 86.04-105.14% and 92.41-110.19%, with the coefficient of variation of 1.71-2.05% and 4.42-5.89% in chicken and pork, respectively.
