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Related Experiment Video

Updated: Oct 8, 2025

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
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In Vitro Non-Genomic Effects of Calcifediol on Human Preosteoblastic Cells.

Simone Donati1, Gaia Palmini1, Cecilia Romagnoli1

  • 1Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy.

Nutrients
|December 28, 2021
PubMed
Summary

Calcifediol [25(OH)D3], a vitamin D3 precursor, rapidly increases intracellular calcium in human adipose-derived mesenchymal stem cells (hADMSCs). This demonstrates calcifediol

Keywords:
calcifediolcalcitriolintracellular Ca2+non-genomic effectsvitamin D3

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Area of Science:

  • Endocrinology
  • Cell Biology
  • Biochemistry

Background:

  • Vitamin D3 precursor, calcifediol [25(OH)D3], binds the vitamin D receptor (VDR) and regulates gene expression.
  • Calcifediol may act as a VDR ligand, potentially activating non-genomic pathways similar to active vitamin D3.
  • Understanding vitamin D's non-genomic effects is crucial for its role in the endocrine system.

Purpose of the Study:

  • To investigate the non-genomic activity of calcifediol [25(OH)D3] in human adipose-derived mesenchymal stem cells (hADMSCs).
  • To assess the ability of 25(OH)D3 to mobilize intracellular calcium (Ca2+) via non-genomic pathways.

Main Methods:

  • In vitro assessment using human adipose-derived mesenchymal stem cells (hADMSCs).
  • Measurement of intracellular Ca2+ mobilization using Fluo-4 AM.
  • Exposure of hADMSCs to 10^-5 M of 25(OH)D3.

Main Results:

  • Calcifediol [25(OH)D3] induced a rapid and sustained increase in intracellular Ca2+ levels in hADMSCs.
  • This calcium mobilization indicates the activation of rapid, non-genomic pathways by 25(OH)D3.
  • The observed effect is comparable to that of the biologically active form of vitamin D3.

Conclusions:

  • Calcifediol [25(OH)D3] demonstrates in vitro ability to activate rapid non-genomic pathways in hADMSCs.
  • This study provides the first evidence of 25(OH)D3 inducing intracellular Ca2+ increase in hADMSCs.
  • Findings contribute to a better understanding of vitamin D3's non-genomic effects and endocrine functions.