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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

585
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
585
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
736

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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
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Micro-Lensed Negative-Curvature Fibre Probe for Raman Spectroscopy.

Karolina Milenko1,2, Stephanos Yerolatsitis3, Astrid Aksnes1

  • 1Department of Electronic Systems, Norwegian University of Science and Technology, O.S. Bragstads Plass 2b, 7034 Trondheim, Norway.

Sensors (Basel, Switzerland)
|December 28, 2021
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Summary
This summary is machine-generated.

Researchers created a tiny micro-lensed fiber probe for Raman spectroscopy. This innovative probe enhances sample collection and is suitable for in vivo applications.

Keywords:
fibre probehollow-core fibreraman sensing

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Area of Science:

  • Optics and Photonics
  • Spectroscopy
  • Biomedical Engineering

Background:

  • Raman spectroscopy is a powerful technique for chemical analysis.
  • Miniaturization of fiber probes is crucial for in vivo applications.
  • Existing fiber probes face challenges with background noise and sample contamination.

Purpose of the Study:

  • To develop a novel miniature micro-lensed fiber probe for enhanced Raman spectroscopy.
  • To minimize Raman background noise and improve collection efficiency.
  • To enable in vivo measurements with a compact and robust probe design.

Main Methods:

  • Fabrication of a probe using a single negative-curvature fiber (NCF) and a micro-lensed fiber cap.
  • Minimizing probe diameter to less than 0.5 mm for enhanced maneuverability.
  • Implementing fiber closure with the cap to prevent sample ingress into hollow fiber structures.

Main Results:

  • Achieved a probe diameter below 0.5 mm, reducing silica-derived Raman background.
  • Demonstrated 1.5-times higher collection efficiency compared to a standard cleaved end-cap.
  • Successfully utilized the probe for measuring varying concentrations of glucose in aqueous solutions.

Conclusions:

  • The novel micro-lensed fiber probe offers a significant advancement for Raman spectroscopy.
  • The probe's design facilitates in vivo measurements with improved sensitivity and reduced background.
  • This technology holds potential for diverse biomedical sensing applications.