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Multiplate platelet aggregometry in dogs undergoing laparoscopic liver biopsy for diagnosis of chronic hepatopathy
S B Shropshire1, C S Olver2, D C Twedt1
1Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA.
Objectives:
To assess platelet function via the Multiplate analyser in dogs undergoing laparoscopic liver biopsy for diagnosis of chronic hepatopathy.
Materials And Methods:
Twenty-seven client-owned dogs were prospectively enrolled. Before laparoscopic liver biopsy, whole blood impedance platelet aggregometry via the Multiplate analyser was performed. Buccal mucosal bleeding time was performed in 23 of 27 dogs. Tissue factor-activated thromboelastography was also performed, in addition to plasma-based coagulation testing. Descriptive statistics were calculated and the prevalence of platelet function abnormalities and results of other biochemical and coagulation testing were reported.
Results:
Seventeen (63%) of 27 dogs had evidence of decreased platelet function as assessed by aggregometry, with all 17 dogs having decreased responsiveness to adenosine diphosphate, and 11 of 17 dogs demonstrating decreased responsiveness to arachidonic acid. Based on maximum amplitude, most dogs were classified as normocoagulable on thromboelastography (15/25; 60%). Other frequent coagulation abnormalities included increased D-dimers (20/27;74%), thrombocytopenia (11/27; 41%), hypofibrinogenemia (4/27; 15%), and decreased antithrombin (4/27; 15%).
Clinical Significance:
Decreased platelet function as assessed by whole blood impedance aggregometry was common in dogs with chronic liver disease. Further study is necessary to determine whether this finding is repeatable or indicative of increased bleeding risk.
