Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

16.7K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
16.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Staphylococcal Cassette Chromosome <i>mec</i> (SCC<i>mec</i>) Natural Excision Frequencies and Its Contributing Factors in Variant SCC<i>mec</i> Type Prototypic Strains.

Antibiotics (Basel, Switzerland)·2026
Same author

Unveiling the overlooked burden of malaria misdiagnosis using lamp-based re-evaluation of routine malaria diagnosis in health centers: implications for public health and clinical practice in Northwest Ethiopia.

BMC infectious diseases·2026
Same author

You Only Look Once (YOLO) based machine learning algorithm for real-time detection of loop-mediated isothermal amplification (LAMP) diagnostics.

PloS one·2026
Same author

Feasibility and impact of pharmacist-led penicillin allergy delabelling using the PEN-FAST scoring tool in a Canadian tertiary care hospital.

Antimicrobial stewardship & healthcare epidemiology : ASHE·2026
Same author

Evaluation of laboratory workers competency on loop mediated isothermal amplification (LAMP) technology for malaria diagnostics: a technology transfer assessment study (LAMP-TTA) during a multi-site clinical trial.

Malaria journal·2025
Same author

Changing prevalence and risk factors for Toxoplasma gondii seropositivity and disease in persons with HIV at the Southern Alberta Clinic since 1985.

HIV medicine·2025

Related Experiment Video

Updated: Oct 8, 2025

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
06:39

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

Published on: April 21, 2022

3.2K

SARS-CoV-2 variant detection with ADSSpike.

Daniel Castañeda-Mogollón1, Claire Kamaliddin1, Laura Fine1

  • 1Cumming School of Medicine, Department of Pathology & Laboratory Medicine, the University of Calgary, Alberta, Canada; Cumming School of Medicine, Department of Microbiology, Immunology, and Infectious Diseases, the University of Calgary, Canada; Calvin, Phoebe & Joan Snyder Institute for Chronic Diseases, the University of Calgary, Calgary, Alberta, Canada.

Diagnostic Microbiology and Infectious Disease
|December 28, 2021
PubMed
Summary

The ADSSpike workflow identifies SARS-CoV-2 variants using S gene signature SNPs via amplicon deep sequencing. This cost-effective tool aids genomic surveillance and diagnosis, helping control COVID-19 transmission.

Keywords:
Amplicon deep sequencingS geneSARS-CoV-2Variants of concernVariants of interest

More Related Videos

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
08:07

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

875
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

4.8K

Related Experiment Videos

Last Updated: Oct 8, 2025

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
06:39

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

Published on: April 21, 2022

3.2K
Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
08:07

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

875
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
10:25

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

4.8K

Area of Science:

  • Virology
  • Genomics
  • Public Health

Background:

  • The COVID-19 pandemic necessitates robust genomic surveillance for emerging SARS-CoV-2 variants.
  • New variants pose challenges to global pandemic response and preparedness.
  • Accurate and timely diagnosis is crucial for reducing viral transmission.

Purpose of the Study:

  • To develop and validate the ADSSpike workflow for identifying Single Nucleotide Polymorphisms (SNPs) in the SARS-CoV-2 Spike (S) gene.
  • To enable practical, cost-effective, and scalable diagnosis of SARS-CoV-2 Variants of Concern (VOC) and Variants of Interest (VOI).

Main Methods:

  • Development of the ADSSpike workflow utilizing amplicon deep sequencing.
  • Sequencing of 72 SARS-CoV-2 samples to identify mutations.
  • Analysis of sequencing data to detect signature SNPs associated with specific variants.

Main Results:

  • Identification of 30 mutations across the sequenced samples.
  • Detection of signature SNPs linked to Zeta-VOI (P.2) and Alpha-VOC (B.1.17) variants.
  • Establishment of an average sequencing depth of 700 reads for accurate SNP and deletion identification.

Conclusions:

  • The ADSSpike workflow provides a practical and cost-effective solution for diagnosing SARS-CoV-2 VOCs/VOIs in clinical laboratories.
  • This workflow enhances genomic surveillance capabilities, supporting public health measures against COVID-19.
  • ADSSpike offers a scalable diagnostic tool at approximately $41.85 USD per reaction.