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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

PCR

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Updated: Oct 8, 2025

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip

Published on: March 29, 2024

[Digital loop-mediated isothermal amplification detection technology and its application progress].

J Huang¹, P Li², S J Shi²

¹Laboratory department,West Hainan Central Hospital, Danzhou 571700,China.

Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine]

|December 28, 2021

Summary

This summary is machine-generated.

Digital loop-mediated isothermal amplification (dLAMP) offers accurate, sensitive nucleic acid quantification. This method shows promise for rapid pathogen detection in clinical, food, and environmental settings.

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Area of Science:

Molecular Biology
Biotechnology
Diagnostics

Background:

Digital loop-mediated isothermal amplification (dLAMP) is an emerging nucleic acid amplification technique.
It enables quantitative detection by partitioning reactions into numerous independent micro-chambers.

Purpose of the Study:

To review the principles, advantages, and applications of dLAMP technology.
To highlight its potential in molecular diagnostics, particularly for pathogen identification.

Main Methods:

Utilizes a highly active chain replacement DNA polymerase and four specific primers.
Performs rapid amplification under isothermal conditions within discrete detection regions.

Main Results:

dLAMP offers high accuracy, sensitivity, and absolute quantification capabilities.
Demonstrates high tolerance to inhibitors and requires simple instrumentation.
Shows significant potential for rapid detection of pathogenic microorganisms.

Conclusions:

dLAMP is a promising tool for molecular diagnostics, clinical applications, food safety, and environmental monitoring.
Challenges include managing non-specific amplification and enabling high-throughput sample analysis.
Further development of dLAMP will significantly advance molecular diagnostics.