Functional traits of poplar leaves and fine roots responses to ozone pollution under soil nitrogen addition

Concurrent ground-level ozone (O₃) pollution and anthropogenic nitrogen (N) deposition can markedly influence dynamics and productivity in forests. Most studies evaluating the functional traits responses of rapid-turnover organs to O₃ have specifically examined leaves, despite fine roots are another major source of soil carbon and nutrient input in forest ecosystems. How elevated O₃ levels impact fine root biomass and biochemistry remains to be resolved. This study was to assess poplar leaf and fine root biomass and biochemistry responses to five different levels of O₃ pollution, while additionally examining whether four levels of soil N supplementation were sufficient to alter the impact of O₃ on these two organs. Elevated O₃ resulted in a more substantial reduction in fine root biomass than leaf biomass; relative to leaves, more biochemically-resistant components were present within fine root litter, which contained high concentrations of lignin, condensed tannins, and elevated C:N and lignin: N ratios that were associated with slower rates of litter decomposition. In contrast, leaves contained more labile components, including nonstructural carbohydrates and N, as well as a higher N:P ratio. Elevated O₃ significantly reduced labile components and increased biochemically-resistant components in leaves, whereas they had minimal impact on fine root biochemistry. This suggests that O₃ pollution has the potential to delay leaf litter decomposition and associated nutrient cycling. N addition largely failed to affect the impact of elevated O₃ levels on leaves or fine root chemistry, suggesting that soil N supplementation is not a suitable approach to combating the impact of O₃ pollution on key functional traits of poplars. These results indicate that the significant differences in the responses of leaves and fine roots to O₃ pollution will result in marked changes in the relative belowground roles of these two litter sources within forest ecosystems, and such changes will independently of nitrogen load.