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Cytotoxic effector function of B lymphoblasts.

L Bersani, F Colotta, G Peri

    Journal of Immunology (Baltimore, Md. : 1950)
    |July 15, 1987
    PubMed
    Summary
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    B lymphoblasts exhibit cytotoxic potential against sarcoma cells, mediated by lymphotoxin. This finding expands our understanding of B cell effector functions in immune responses.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Mononuclear phagocytes were previously identified as effector cells in the WEHI 164 sarcoma cell killing system.
    • Resting B cells lack significant cytolytic activity.

    Purpose of the Study:

    • To investigate the cytotoxic potential of B lymphoblasts.
    • To determine the mechanism underlying B lymphoblast-mediated cytotoxicity.

    Main Methods:

    • Utilized EBV-transformed B lymphoblastoid cell lines and normal B lymphoblasts.
    • Assessed cytotoxicity against Actinomycin D-pretreated WEHI 164 sarcoma cells.
    • Investigated the role of lymphotoxin and tumor necrosis factor using specific antisera.

    Main Results:

    • EBV-transformed B lymphoblastoid cell lines demonstrated in vitro cytotoxicity against WEHI 164 cells.

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  • Activated normal B lymphoblasts also exhibited significant cytolytic activity.
  • Cytotoxicity was inhibited by anti-lymphotoxin but not anti-tumor necrosis factor antiserum, indicating lymphotoxin mediation.

    • Conclusions:

    • B lymphoblasts possess cytotoxic potential.
    • This cytotoxicity is mediated by lymphotoxin or a similar soluble product.
    • B cells can function as effector cells in cell-mediated killing.