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Antigenic phenotype of NIH 3T3 cell line
Journal of the National Cancer Institute
|July 1, 1987
Summary
NIH 3T3 cells possess a unique antigenic phenotype, including H-2q, Qa-2, and Ly-6.2 antigens. This characterization aids in selecting compatible mouse strains, like B10.Q or DBA/1, for in vivo transplantation experiments.
Area of Science:
- Immunology
- Cell Biology
- Genetics
Background:
- NIH 3T3 is a widely used immortalized mouse cell line.
- Understanding its antigenic profile is crucial for experimental applications, particularly in transplantation studies.
- The cell line originates from NIH Swiss mice, which are outbred, complicating syngeneic transplantation.
Purpose of the Study:
- To comprehensively determine the antigenic phenotype of the NIH 3T3 cell line.
- To identify cell surface antigens using monoclonal antibodies and alloantisera.
- To inform the selection of appropriate recipient mice for in vivo studies involving NIH 3T3 cells.
Main Methods:
- Utilized a panel of monoclonal antibodies and alloantisera targeting H-2 and non-H-2 antigens.
- Employed complement-dependent microcytotoxicity assays to detect antibody binding.
- Quantified cell surface antigen expression via indirect immunofluorescence and flow cytometry.
Main Results:
- NIH 3T3 cells exhibited the antigenic phenotype: H-2q, Qa-2, Ly-6.2, Thy-1.2, Ly-23.2, and 9F 3+.
- Expression levels of H-2 antigens (Kq, Dq/Lq) were lower than in B10.Q (H-2q) T-lymphocytes; Qa-2 expression was very low.
- Strong expression of Ly-m6.2A was observed, while Ly-m.6B, Ly-m.6C, ThB, and H9/25 were undetected.
Conclusions:
- The determined antigenic phenotype of NIH 3T3 cells provides a detailed molecular fingerprint.
- The lack of a syngeneic recipient necessitates careful selection of immunocompetent hosts for in vivo experiments.
- Inbred mouse strains B10.Q and DBA/1 are identified as potentially compatible recipients for NIH 3T3 cell transplantation based on shared antigens.