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Updated: Oct 8, 2025

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
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P2X7 receptor: the regulator of glioma tumor development and survival

Damian Matyśniak1, Vira Chumak1,2, Natalia Nowak3

  • 1Laboratory of Molecular Basis of Cell Motility, Nencki Institute of Experimental Biology of Polish Academy of Sciences, 3 Pasteur Str., 02-093, Warsaw, Poland.

Purinergic Signalling
|December 29, 2021
PubMed

Insights

The P2X7 receptor promotes glioma cell growth and survival. Blocking P2X7 with Brilliant Blue G inhibits tumor development and alters immune response, suggesting P2X7 as a therapeutic target for glioma.

Area of Science:

  • Neuroscience
  • Oncology
  • Cell Biology

Background:

  • P2X7 receptor (P2X7) is an ionotropic nucleotide receptor involved in cell signaling.
  • While P2X7 activity is known in somatic cells, its role in glioma tumors is understudied.

Purpose of the Study:

  • To comprehensively investigate the role and activity of P2X7 in glioma biology.
  • To evaluate P2X7 as a potential therapeutic target in glioma.

Main Methods:

  • In vitro studies using C6 and glioma cell lines.
  • In vivo studies involving glioma tumor models.
  • Treatment with P2X7 antagonist Brilliant Blue G.
  • Microarray analysis of human glioma patient data.

Main Results:

  • P2X7 stimulation enhanced glioma cell proliferation, viability, adhesion, and reactive oxygen species production.
  • P2X7 activation promoted glioma tumor growth in vivo via pro-survival pathways and ATP release.
  • Brilliant Blue G treatment inhibited tumor development, reduced negative prognostic markers, and modulated immune response.
  • Human glioma tissues (grades I-III) showed P2X7 receptor upregulation.

Conclusions:

  • P2X7 receptor plays a significant role in glioma biology, promoting tumor growth and survival.
  • Targeting P2X7 with antagonists like Brilliant Blue G shows therapeutic potential for glioma treatment.
  • P2X7 modulation impacts tumor progression, prognostic markers, and immune response in glioma.
Keywords:
Cell survivalGliomaP2X7ROS signaling

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