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UBC Mediated by SEPT6 Inhibited the Progression of Prostate Cancer

Ruochen Zhang1, Yaojing Yang1, Haijian Huang1

  • 1Shengli Clinical Medical College of Fujian Medical University, Department of Urology, Fujian Provincial Hospital, China.

Mediators of Inflammation
|December 30, 2021
PubMed
Abstract

Insights

SEPT6 inhibits prostate cancer progression by downregulating UBC expression, reducing ubiquitination, and affecting cell proliferation. This study identifies SEPT6 and UBC as potential therapeutic targets for prostate cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Prostate cancer is a prevalent malignancy in men.
  • Protein ubiquitination is a key regulator of protein activity and levels.
  • Understanding the roles of SEPT6 and UBC in prostate cancer is crucial for identifying new therapeutic targets.

Purpose of the Study:

  • To investigate the mechanistic roles of SEPT6 and UBC in prostate cancer.
  • To explore the relationship between SEPT6 and UBC in prostate cancer progression.
  • To identify potential therapeutic targets for prostate cancer treatment.

Main Methods:

  • Detected expression of ubiquitin-protein and ubiquitin-coding genes (UBA52, UBA80, UBB, UBC) in clinical tissues and cells.
  • Performed overexpression and knockdown experiments of UBC and SEPT6 in DU145 prostate cancer cells.
  • Utilized CCK-8, flow cytometry, and clonal formation assays to assess cell proliferation and cell cycle.
  • Employed immunofluorescence for colocalization studies and Pearson correlation analysis for relationship assessment.
  • Conducted tumor formation experiments to evaluate the in vivo effect of SEPT6/UBC.

Main Results:

  • UBC expression was found to be upregulated in prostate cancer tissues and cells.
  • Overexpression of UBC enhanced prostate cancer cell survival and proliferation.
  • SEPT6 was identified as an upstream regulator of UBC, with SEPT6 inhibiting UBC expression.
  • SEPT6 demonstrated an inhibitory effect on tumor growth in vivo.
  • A negative correlation was observed between SEPT6 and UBC expression in prostate cancer tissues.

Conclusions:

  • SEPT6 inhibits prostate cancer progression by suppressing UBC expression.
  • This inhibition leads to reduced overall ubiquitination and affects the expression of downstream proliferation-related genes.
  • SEPT6 acts as a tumor suppressor in prostate cancer, potentially through its regulation of UBC.

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