Hepatic effects of acrylamide in rainbow trout

Acrylamide effects on hepatic mixed function oxidase activity were examined in rainbow trout exposed to waterborne acrylamide monomer (0-50 mg/liter) for 14 days. Acrylamide doses of 25 mg/liter and higher produced reversible dose-related histological lesions in the liver characterized by necrosis around the central vein. A profound selective decrease in ethoxyresorufin-O-deethylase activity was observed in hepatic microsomes from acrylamide-treated trout. However, in vitro preincubation of acrylamide with untreated rainbow trout hepatic microsomes with or without NADPH did not produce a similar decrease in ethoxyresorufin-O-deethylase activity. Structural analysis of a trout biliary metabolite of acrylamide, although not identified, revealed the presence of primary alcohol, carboxylic acid, and amide carbonyl functional groups.