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Multipronged Phenotyping Approaches to Characterize Sugarcane Root Systems
Published on: August 17, 2022
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Multi-dimensional leaf phenotypes reflect root system genotype in grafted grapevine over the growing season
Zachary N Harris1,2, Mani Awale3, Niyati Bhakta1,2
1Department of Biology, Saint Louis University, 3507 Laclede Avenue, St. Louis, MO 63103-2010, USA.
Gigascience
|December 30, 2021
Summary
Rootstock genotype subtly influences grapevine shoot phenotypes across multiple measurements, with the strongest effects seen in leaf ion composition. This rootstock impact on the scion is dynamic throughout the growing season.
Area of Science:
- Plant biology
- Genetics
- Agricultural science
Background:
- Grafting is a key technique to study rootstock-scion interactions.
- Rootstock influence on grapevine scion physiology and berry chemistry is known.
- Rootstock effects on leaf phenotypes and their seasonal changes are poorly understood.
Purpose of the Study:
- Investigate rootstock genotype effects on shoot system phenotypes in grapevines.
- Quantify rootstock influence across multiple leaf phenotyping modalities.
- Determine the seasonal dynamics of rootstock-scion interactions.
Main Methods:
- Utilized grafting to create distinct rootstock-scion combinations.
- Applied five multi-dimensional phenotyping techniques: ionomics, metabolomics, transcriptomics, morphometrics, and physiology.
- Collected data across the entire growing season.
Main Results:
- Rootstock genotype ubiquitously, yet subtly, influences scion phenotypes across all measured modalities.
- The leaf ionome exhibits the most pronounced signature of rootstock influence.
- Rootstock effects on scion phenotypes and their covariation patterns are highly dynamic throughout the season.
Conclusions:
- Rootstock influence on scion phenotypes is complex and changes over time.
- Comprehensive understanding requires multi-dimensional data and seasonal analysis.
- This study provides a detailed view of rootstock-scion interactions in grapevines.
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