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Related Experiment Video

Updated: Oct 8, 2025

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

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Membrane-Based In-Gel Loop-Mediated Isothermal Amplification (mgLAMP) System for SARS-CoV-2 Quantification in

Yanzhe Zhu¹, Xunyi Wu¹, Alan Gu¹

¹Linde Laboratories, California Institute of Technology, Pasadena, California 91125, United States.

Environmental Science & Technology

|December 30, 2021

Summary

This summary is machine-generated.

A new membrane-based in-gel loop-mediated isothermal amplification (mgLAMP) system rapidly quantifies severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in water. This method offers a sensitive and accessible tool for environmental surveillance of the virus.

Keywords:

RT-LAMP SARS-CoV-2 environmental quantification of SARS-CoV-2 in milliliters of environmental water samples hydrogel membrane

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Area of Science:

Environmental science
Molecular biology
Biotechnology

Background:

The COVID-19 pandemic's transition to endemic status necessitates robust environmental surveillance for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Accurate quantification of SARS-CoV-2 in ambient waters is crucial for early outbreak detection and public health monitoring.

Purpose of the Study:

To develop a rapid, point-of-use system for quantifying SARS-CoV-2 in environmental water samples.
To create a user-friendly method suitable for large-scale environmental surveillance without specialized equipment or extensive training.

Main Methods:

Development of a membrane-based in-gel loop-mediated isothermal amplification (mgLAMP) system.
Integration of viral concentration, in-assay lysis, and hydrogel-based RT-LAMP quantification with enhanced fluorescence detection.
Utilization of a 3D-printed portable device for incubation and fluorescence illumination, coupled with smartphone imaging and machine learning for result interpretation.

Main Results:

The mgLAMP system achieved a sample-to-result time of under 1 hour.
Detection of SARS-CoV-2 below 0.96 copies/mL in Milli-Q water.
In surface water, mgLAMP detected down to 93 copies/mL, outperforming RT-qPCR which was inhibited at 930 copies/mL.

Conclusions:

The mgLAMP system demonstrates high sensitivity and efficiency for SARS-CoV-2 quantification in environmental waters.
This method is a promising tool for accessible, large-scale environmental surveillance of SARS-CoV-2.
The portable, user-friendly design facilitates widespread adoption in public health monitoring efforts.