Related Experiment Video
Updated: Aug 25, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells
Published on: June 23, 2014
Antibodies against microfibrils of developing connective tissue in patients with inflammatory conditions
Abstract:
Sera from patients with chronic inflammatory disease were tested for the presence of connective tissue antibodies. Fetal human skin was used as a substrate in an indirect immunofluorescence assay. Histological staining for connective tissue fibres, antibodies to connective tissue components and immunoelectron microscopy were used to identify the antigenic structures. Some antigenic fibrils were associated with elastin. By immunoelectron microscopy antigenic extracellular microfibrillar structures were identified. Antibodies against these microfibrils were detected in higher titres only in chronic inflammation (14%). Our results suggest that antibodies against connective tissues microfibrils may occur in human sera.
More Related Videos
06:35An Immunohistopathologic Study to Profile the Folate Receptor Beta Macrophage and Vascular Immune Microenvironment in Giant Cell Arteritis
Published on: February 8, 2019
09:43Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
Published on: June 8, 2022
Related Concept Videos
Amyloid Fibrils
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining, normally used to...
Connective Tissue Cell Types
Fat cells (adipocytes), smooth muscle cells (myoblasts), and bone cells (osteoblasts) are some connective tissue cell types. Some immune system cells...
Fibril-associated Collagen
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF
Chronic Inflammation: Introduction
Inflammatory Bowel Disease III: Crohn's Disease