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Berberine inhibits gastric cancer development and progression by regulating the JAK2/STAT3 pathway and downregulating

Minmin Xu1, Li Ren1, Jinhua Fan1

  • 1Engineering Research Center of Coptis Development & Utilization (Ministry of Education), School of Life Sciences, Southwest University, Chongqing 400715, China.

Life Sciences
|December 30, 2021
PubMed
Abstract

Insights

Berberine (BBR) effectively inhibits gastric cancer (GC) cell proliferation, migration, and invasion. This natural compound shows promise for gastric cancer prevention by targeting the IL-6/JAK2/STAT3 pathway.

Area of Science:

  • Oncology
  • Pharmacology
  • Natural Products

Background:

  • Gastric cancer (GC) is a significant global health concern.
  • Berberine (BBR), a natural alkaloid, exhibits anti-cancer properties.
  • Understanding BBR's mechanism in GC is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the anti-cancer effects of Berberine (BBR) on gastric cancer (GC) cells.
  • To elucidate the underlying molecular mechanisms of BBR's action in GC.
  • To evaluate BBR's potential for gastric cancer prevention.

Main Methods:

  • In vitro assays: MTT, flow cytometry, scratch, and Transwell assays were used to assess proliferation, apoptosis, cell cycle arrest, migration, and invasion.
  • In vivo studies: Xenograft nude mouse models were employed to evaluate tumor growth inhibition.
  • Molecular analyses: RNA-Seq, qRT-PCR, Western Blot (WB), and ELISA were utilized to explore signaling pathways.

Main Results:

  • BBR inhibited proliferation and induced apoptosis and G0/G1 cell cycle arrest in MKN-45 and HGC-27 gastric cancer cells.
  • BBR suppressed gastric cancer cell migration and invasion in vitro and inhibited tumor growth in vivo.
  • RNA-Seq identified IL-6 as a key target, with BBR modulating the IL-6/JAK2/STAT3 signaling pathway.

Conclusions:

  • Berberine demonstrates significant anti-neoplastic effects against gastric cancer cells in vitro and in vivo.
  • BBR exerts its effects by targeting the IL-6/JAK2/STAT3 signaling pathway.
  • This study provides a scientific foundation for exploring BBR in gastric cancer prevention strategies.
Keywords:
BerberineGastric cancerIL-6JAK2/STAT3RNA-sequencing

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