Online evaluation of bacterial cells in sand filter effluents during full-scale treatment of drinking water

Ensuring the microbiological safety of drinking water is critical to protect public health. This study aimed to evaluate the reliability of real-time bacteriological counter coupled with an online dialysis membrane-based pre-treatment system for continuously monitoring bacterial cell counts in sand filter effluents of a full-scale drinking water treatment plant. The pre-treatment system, which included anion exchange resins (porous polymeric microbeads that trap ions for releasing other ions) for dialysate regeneration, successfully achieved the stable attenuation of background interfering substances (humic acids) during the 19-d test. The real-time bacteriological counter equipped with the pre-treatment system provided a continuous profile of bacterial cell counts in the sand filter effluent (0.2-2.5 × 10⁴ counts/mL). The online analysis identified different timing of concentration peaks between particle and bacterial cell counts after backwashing. Bacterial community analysis revealed that Proteobacteria, Planctomycetes, and Cyanobacteria were the dominating phyla. Further, total bacterial cell counts determined by fluorescence microscopy and SYBR® Green I staining, a commonly accepted parameter, was found to be an indicator of online-monitored bacterial cell counts. The results indicated the potential of monitoring the bacterial cell counts in a sand filter process for providing an early warning of filter failures, which can allow plant operators to diagnose the overall system and provide countermeasures.