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Extraction method development for nanoplastics from oyster and fish tissues.

Yu-Shan Chang1, Shih-Hsuan Chou2, Ya-Jhu Jhang3

  • 1Master Program in Food Safety, College of Nutrition, Taipei Medical University, Taipei 10031, Taiwan.

The Science of the Total Environment
|December 30, 2021
PubMed
Summary

A new method efficiently extracts nanoplastics from seafood like oysters and fish. This technique uses enzymatic digestion and filtration, paving the way for better nanoplastic detection in human food sources.

Keywords:
Enzymatic digestionNanoparticle tracking analysisNanoplasticsPVCRecovery

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Food Safety

Background:

  • Nanoplastics are increasingly detected in environmental media and consumer products.
  • Limited data exists on nanoplastics in seafood, a primary human consumption source.
  • A standardized method for nanoplastic extraction from seafood is currently lacking.

Purpose of the Study:

  • To develop and validate an efficient method for extracting nanoplastics from oyster and fish tissues.
  • To assess the effectiveness of enzymatic digestion and purification techniques for nanoplastic isolation.
  • To establish a pretreatment protocol for nanoplastic detection in seafood.

Main Methods:

  • Enzymatic digestion (Corolase, lipase) of seafood tissues.
  • Sequential membrane filtration and centrifugal concentration.
  • Purification using dialysis and sodium dodecyl sulfate (SDS) incubation.
  • Nanoparticle Tracking Analysis (NTA) for validation.

Main Results:

  • Corolase and lipase treatment achieved high digestion efficiencies (88-89%) for non-homogenized tissues.
  • Extraction procedures showed good recoveries (71-110%) for labeled nanoplastics (PVC, PS).
  • Enzymatic digestion preserved the morphology of most nanoplastic types (PVC, PE, PS).
  • Method blanks contained minimal residual particles (150-300 nm).

Conclusions:

  • An efficient method for nanoplastic extraction from seafood was successfully developed.
  • The method utilizes common techniques and demonstrates high digestion efficiency and recovery rates.
  • This protocol serves as a crucial pretreatment step for future nanoplastic detection in seafood.