Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

183
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
183

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Clinical Case of Treatment-Emergent Acinetobacter baumannii Non-Susceptibility to Sulbactam-Durlobactam.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026
Same author

Time to Effective Therapy and Mortality in Community-Acquired Legionella Pneumonia: A Multicenter Retrospective Cohort Study.

Annals of the American Thoracic Society·2026
Same author

Fluorogenic/chromogenic enzyme substrates constructed around the 2-amino-7-nitrofluorene core structure for the detection of aminopeptidase activities in clinically important microorganisms.

Bioorganic chemistry·2026
Same author

Environmental reservoirs of carbapenem-resistant organisms in the intensive care unit: a multicenter longitudinal study in two middle-income country hospitals.

Antimicrobial resistance and infection control·2026
Same author

<i>Legionella</i> 5S rRNA PCR melting temperature analysis discriminates high-risk species associated with disease severity.

Journal of clinical microbiology·2026
Same author

Comparison of laboratory-developed methods for aztreonam plus ceftazidime-avibactam antimicrobial susceptibility testing for metallo-beta-lactamase-producing Enterobacterales.

Journal of clinical microbiology·2026

Related Experiment Video

Updated: Oct 8, 2025

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

2.9K

Recent Advances in Rapid Antimicrobial Susceptibility Testing.

Rucha Datar1, Sylvain Orenga1, Romain Pogorelcnik2

  • 1bioMérieux, Microbiology Research, La Balme Les Grottes, France.

Clinical Chemistry
|December 30, 2021
PubMed
Summary

Traditional antimicrobial susceptibility testing (AST) relies on bacterial growth. Newer methods offer faster results for antimicrobial resistance detection but require further implementation for routine clinical use.

More Related Videos

Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles
09:07

Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles

Published on: May 24, 2024

1.1K
One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
08:30

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

25.7K

Related Experiment Videos

Last Updated: Oct 8, 2025

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

2.9K
Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles
09:07

Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles

Published on: May 24, 2024

1.1K
One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
08:30

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

25.7K

Area of Science:

  • Clinical microbiology
  • Infectious diseases
  • Antimicrobial resistance

Background:

  • Classical antimicrobial susceptibility testing (AST) methods depend on visible bacterial growth.
  • Growth-based techniques require viable bacteria for detection, limiting speed and throughput.
  • Traditional AST has seen minimal evolution in accuracy and rapidity over the 20th century.

Purpose of the Study:

  • To review novel methods for antimicrobial susceptibility testing.
  • To identify requirements for implementing advanced AST in clinical practice.
  • To bridge the gap between emerging technologies and routine antimicrobial resistance detection.

Main Methods:

  • Review of nucleic acid-mediated amplification technologies.
  • Analysis of proteomic and other "omic" methodologies for AST.
  • Evaluation of next-generation sequencing and single-cell visualization techniques.

Main Results:

  • Recent advancements include nucleic acid amplification, "omics" approaches, and next-generation sequencing.
  • These novel methods have the potential to significantly increase AST rapidity.
  • Single-cell visualization techniques have been developed but are not yet widely adopted.
  • Current high-throughput clinical detection of antimicrobial resistance has not been substantially altered by these new technologies.

Conclusions:

  • New generation AST methods offer enhanced speed and potential for improved antimicrobial resistance detection.
  • Urgent needs exist for the implementation of these advanced AST methods into daily clinical practice.
  • Further integration is crucial for effective management of infectious diseases and antimicrobial resistance.