Increased yields of IL-2 in media conditioned by MLA 144 cells

The gibbon leukaemia cell line MLA 144 produces IL-2 constitutively, and has been used as a source of IL-2 for the in vitro growth of T cells from a range of species. We have tried to increase the amount of IL-2 present in conditioned medium produced by this cell line, by manipulating the growth conditions and using compounds known to increase IL-2 production in other cell lines. We found that conditioned media containing a higher amount of IL-2 could be obtained from 72 h cultures of MLA 144 cells originally seeded at 4 X 10(5) cells/ml in DMEM containing 10% FBS and supplemented with 2-ME at 5 X 10(-5) M and PMA at 10 ng/ml.