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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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RNA Splicing01:32

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Meiosis II02:02

Meiosis II

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Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
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Chromatin Structure and RNA Splicing02:41

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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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BCAS2 is involved in alternative splicing and mouse oocyte development.

Jiaqi Zhang1,2, Wenbo Liu1,3,4, Guangyue Li5

  • 1State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|December 31, 2021
PubMed
Summary

Breast carcinoma amplified sequence 2 (BCAS2) is crucial for mouse oocyte development and fertility. Its deletion impairs oogenesis and follicular growth by altering alternative splicing events via the PRP19 complex.

Keywords:
BCAS2PRP19 complexalternative splicingfollicleoogenesis

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Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
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Area of Science:

  • Reproductive biology
  • Molecular genetics
  • Cellular biology

Background:

  • Alternative splicing (AS) regulates organogenesis and fertility.
  • Breast carcinoma amplified sequence 2 (BCAS2) is a core component of the PRP19 complex, influencing meiosis initiation in male mice.
  • The role of BCAS2 in mouse oogenesis is largely unknown.

Purpose of the Study:

  • To investigate the function of BCAS2 in mouse oogenesis.
  • To determine the impact of BCAS2 deletion on oocyte quality and follicular development.

Main Methods:

  • Utilized Vasa-Cre-mediated deletion to remove Bcas2 in mouse oocytes.
  • Analyzed alternative splicing events and gene expression changes.
  • Examined oocyte quality, oogenesis, and follicular development.
  • Assessed the integrity of PRP19 core proteins.

Main Results:

  • BCAS2 is highly expressed in primordial follicle oocytes.
  • Bcas2 deletion resulted in poor oocyte quality, abnormal oogenesis, and follicular development.
  • Deletion altered 991 AS events in 706 genes, including those related to oogenesis and spindle assembly (e.g., Pabpc1l, Nobox, Zfp207, Mybl2, Prc1, Spc25).
  • BCAS2 disruption led to the degradation of PRP19 core proteins in oocytes.

Conclusions:

  • BCAS2 plays a critical role in mouse oocyte development.
  • BCAS2 regulates functional gene alternative splicing through the PRP19 complex during oogenesis.
  • Disruption of BCAS2 impacts oocyte quality and follicular development, highlighting its importance in female fertility.