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Updated: Oct 8, 2025

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
08:40

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice

Published on: November 22, 2017

17.8K

Induction and Detection of Mitophagy

Maria A Yapryntseva1, Boris Zhivotovsky1,2, Vladimir Gogvadze3,4

  • 1Faculty of Basic Medicine, Lomonosov Moscow State University, Moscow, Russia.

Methods in Molecular Biology (Clifton, N.J.)
|January 1, 2022
PubMed

Insights

Mitophagy removes damaged mitochondria for cellular health. This study details methods to investigate this vital mitochondrial quality control process, crucial for preventing disease.

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Autophagy Research

Background:

  • Mitophagy is a key mitochondrial quality control mechanism.
  • It involves selective elimination of damaged mitochondria via autophagy.
  • Impairment of mitophagy is linked to various pathologies.

Purpose of the Study:

  • To describe methods for investigating mitophagy.
  • To provide tools for studying mitochondrial quality control.

Main Methods:

  • General autophagy induction.
  • Priming of damaged mitochondria for autophagic recognition.
  • Investigative techniques for mitophagy.

Main Results:

  • Established methodologies for mitophagy investigation.
  • Detailed protocols for studying mitochondrial clearance.

Conclusions:

  • Investigating mitophagy is essential for understanding cellular health.
  • These methods aid in studying pathologies linked to mitophagy defects.
Keywords:
AutophagyConfocal microscopyFlow cytometryMitochondriaMitophagyRespiration

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