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Updated: Oct 8, 2025
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On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
Published on: May 31, 2020
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Methodological Approaches for Assessing Metabolomic Changes in Glioblastomas.
Trang T T Nguyen1, Enyuan Shang2, Mike-Andrew Westhoff3
1Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 1, 2022
Summary
Glioblastoma metabolism reprogramming by IDH mutations and oxidative pathways is key. Fatty acid metabolism is a novel therapeutic target for this aggressive brain cancer.
Area of Science:
- Neuro-oncology
- Cancer Metabolism
- Molecular Biology
Background:
- Glioblastoma (GBM) is a fatal brain tumor with evolving molecular insights.
- IDH1/IDH2 mutations reprogram GBM metabolism via oncometabolite production.
- Oxidative metabolism and metabolic zoning are critical GBM hallmarks.
Purpose of the Study:
- To review key findings in glioblastoma metabolism and cell death.
- To explore how metabolic alterations influence GBM progression and therapy response.
- To summarize current methods for measuring metabolic changes in GBM.
Main Methods:
- Review of recent literature on GBM metabolism.
- Analysis of IDH mutation impact on cellular metabolism.
- Discussion of metabolic zoning and fatty acid metabolism in GBM.
- Overview of metabolomic analysis techniques (labeled/unlabeled metabolites, extracellular flux).
Main Results:
- IDH mutations confer an oncometabolite, reprogramming GBM metabolism.
- Oxidative metabolism plays a more significant role than previously thought.
- Enrichment of fatty acid derivatives at the infiltrative margin suggests therapeutic potential.
- Metabolic interplay is crucial for GBM progression and treatment resistance.
Conclusions:
- Understanding GBM metabolism, including IDH mutations and fatty acid pathways, is vital.
- Metabolic reprogramming offers novel therapeutic targets for glioblastoma.
- Advanced metabolomic techniques are essential for studying GBM.
