Related Experiment Video
Updated: Oct 8, 2025
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Published on: September 20, 2016
An effective strategy for improving the specific activity and saccharification efficiency of cellulase by
Qiuping Ran1, Huanan Li1, Jiashu Liu1
1State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan 430062, PR China; Hubei Key Laboratory of Industrial Biotechnology, School of Life Science, Hubei University, Wuhan 430062, PR China.
Abstract:
This short communication analyzed the effects of lignin-derived phenolic acid compounds on cellulase. Vanillic acid, syringic acid, ferulic acid, and isovanillic acid improved cellulase specific activity and saccharification efficiency. In the enzymatic hydrolysis process, the promotion effect of phenolic acid was concentration-dependent. The effect of low concentration of phenolic acids (less than 5 mM) was negligible. After pre-incubating 1 g cellulase with 5 mmol phenolic acid, FPase-specific activity, CMCase-specific activity, and pNPGase-specific activity increased by 57.06%, 136.79%, and 110.61%, respectively. After digestion with pre-incubated cellulase, the saccharification efficiency of phosphoric acid-swollen cellulose increased by 45.13%. Pre-incubation with phenolic acid improved the saccharification efficiency of cellulase. It might be helpful to enhance the comprehensive utilization capacity of lignin-derived compounds.
