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Updated: Oct 8, 2025

08:13
A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
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Combining continuous flow aerobic granulation using an external selector and carbon-efficient nutrient removal with
Pusker Regmi1, Belinda Sturm2, Dev Hiripitiyage2
1Brown and Caldwell, Walnut Creek, CA, USA.
Water Research
|January 1, 2022
Summary
Hydrocyclone-based wasting and AvN aeration controls significantly improved wastewater treatment at the Pueblo, Colorado facility. This approach enhanced settling, reduced energy use by 50%, and achieved excellent nutrient removal.
Area of Science:
- Environmental Engineering
- Microbiology
- Wastewater Treatment
Background:
- The James R. Dolorio Water Reclamation Facility sought to optimize energy efficiency and nutrient removal.
- Traditional methods faced challenges in sludge settling and process intensification.
Purpose of the Study:
- To evaluate the effectiveness of AvN aeration controls and hydrocyclone-based wasting for improved wastewater treatment.
- To assess the impact on aeration energy, nutrient removal, and sludge settling characteristics (SVI).
Main Methods:
- Implementation of AvN aeration controls for low dissolved oxygen (DO) conditions.
- Utilized hydrocyclone-based wasting to selectively remove lighter microbial fractions.
- Conducted molecular analysis to identify microbial communities, including comammox.
- Performed ex-situ batch tests to identify denitrifying phosphorus-accumulating organisms (DPAO).
Main Results:
- Sludge Volume Index (SVI) improved from 200 ± 52 mL/g to 83 ± 22 mL/g.
- Aeration energy demand was reduced by 50% under low DO conditions (< 0.3 mgO2/L).
- Achieved excellent effluent nitrogen (TIN < 11 mgN/L) and phosphorus (TP < 1 mgP/L) removal.
- Demonstrated preferential retention of PAO and nitrifiers in dense aggregates.
Conclusions:
- AvN aeration controls and hydrocyclone-based wasting offer a carbon-efficient solution for nutrient removal and process intensification.
- Selective wasting uncoupled the sludge retention time (SRT) of different microbial populations, enhancing settling.
- The presence of comammox and DPAO likely contributed to the observed efficient biological nutrient removal.

