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Polyprotic Acids

Polyprotic Acids

Acids are classified by the number of protons per molecule that they can give up in a reaction. Acids such as HCl, HNO3, and HCN that contain one ionizable hydrogen atom in each molecule are called monoprotic acids. Their reactions with water are:

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Related Experiment Video

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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

A highly sensitive switch-on spectrofluorometric method for determination of ascorbic acid using a selective

Samah F El-Malla¹, Rehab H Elattar¹, Amira H Kamal¹

¹Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta 31111, Egypt.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|January 1, 2022

Summary

A new, highly sensitive spectrofluorometric method determines ascorbic acid by enhancing salicylate fluorescence. This greener approach offers a simple, rapid alternative for analyzing ascorbic acid in various samples.

Keywords:

Ascorbic acid Iron (III) salicylate Redox reaction Spectrofluorometry

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Area of Science:

Analytical Chemistry
Biochemistry

Background:

Ascorbic acid (vitamin C) is crucial for immune support, particularly noted during COVID-19 management.
Existing spectrofluorometric methods for ascorbic acid analysis have limitations like slow kinetics, low sensitivity, and harsh conditions.

Purpose of the Study:

To develop a highly sensitive, rapid, and greener spectrofluorometric method for determining ascorbic acid.
To validate the method for analyzing ascorbic acid in pharmaceutical formulations.

Main Methods:

A novel 'turn-on' spectrofluorometric approach utilizing the fluorescence enhancement of a salicylate-iron (III) complex.
Ascorbic acid reduces iron (III) to iron (II), leading to increased fluorescence intensity at 411 nm.
Method validation included linearity, sensitivity (LOD/LOQ), and application to commercial tablets (Ruta C60®).

Main Results:

The method demonstrated excellent linearity (R²=0.9997) in the 1-8 µg/mL range.
Achieved low limits of detection (0.035 µg/mL) and quantitation (0.106 µg/mL).
Results for pharmaceutical samples showed no significant difference compared to a liquid chromatography method and were markedly greener.

Conclusions:

A simple, rapid, and sensitive spectrofluorometric method for ascorbic acid determination was successfully developed.
The method is validated for pharmaceutical analysis and exhibits superior environmental performance compared to existing techniques.
Potential applications include analysis in pharmaceuticals, biological fluids, and food samples.