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Tunable particle/cell separation across aqueous two-phase system interface by electric pulse in microfluidics.

Mengqi Li1, Deyu Li1, Yongxin Song1

  • 1Department of Marine Engineering, Dalian Maritime University, Dalian 116026, China.

Journal of Colloid and Interface Science
|January 2, 2022
PubMed
Summary

Aqueous two-phase system (ATPS) electrophoresis in microfluidics enables tunable particle and cell separation. This method uses electric pulses for precise sorting, purification, and viability identification, advancing biomedical research and environmental assessment.

Keywords:
Aqueous two-phase systemElectrophoresisMicroalgae sortingMicrofluidic chipParticle separation

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Area of Science:

  • Microfluidics
  • Biotechnology
  • Analytical Chemistry

Background:

  • Particle and cell separations are crucial in microfluidic systems for chemistry and biomedicine.
  • Aqueous two-phase system (ATPS) interfaces can function as selective liquid filters.
  • Electric field stimuli can induce targeted transfer across liquid-liquid interfaces for separation.

Purpose of the Study:

  • To investigate particle and cell separations using ATPS electrophoresis in a microfluidic chip.
  • To systematically study the mechanism of ATPS electrophoresis.
  • To demonstrate the separation of particles and microalgae cells.

Main Methods:

  • Utilized microfluidic chip-based ATPS electrophoresis.
  • Investigated polystyrene (PS) particles to understand ATPS electrophoresis mechanisms.
  • Demonstrated separation of particles and microalgae cells.

Main Results:

  • Electrophoretic transfer across the ATPS interface depends on electric pulse strength, particle size, zeta potential, and hydrophobicity.
  • Achieved continuous particle and cell separation via controllable interfacial transfer using electric pulses.
  • Demonstrated tunable separation for particle/cell separation, cell purification, and viability identification by adjusting electric pulse magnitude.

Conclusions:

  • Tunable ATPS electrophoresis offers multidimensional particle and cell sorting capabilities.
  • This technique has potential applications in microorganism seed selection, environmental assessment, and biomedical research.
  • The ability to control particle and cell transfer across the ATPS interface provides a versatile separation platform.