Influence of probiotic bacteria on gut microbiota composition and gut wall function in an in-vitro model in patients...

Area of Science:

• Microbiology
• Gastroenterology
• Neuroscience

Background:

• Parkinson's disease (PD) is associated with gut microbiota dysbiosis.
• Altered bacterial phyla, including increased Firmicutes and reduced Bacteroidetes, are observed in PD patients.
• Gut health may play a role in the development and progression of PD.

Purpose of the Study:

• To investigate the effects of a 4-strain probiotic suspension on gut microbiota composition and function in Parkinson's disease (PD) patients.
• To evaluate the impact of probiotic dosing on gut barrier integrity, inflammation, and wound healing in cell culture models.

Main Methods:

• An in-vitro gut model was established using stool samples from PD patients and healthy controls.
• The model was dosed with an oral probiotic suspension (Symprove) for 48 hours.
• Cell culture models were used to assess epithelial tight-junction integrity, inflammatory marker production, and wound healing.

Main Results:

• Probiotic dosing altered bacterial composition in the PD patient microbiotas.
• Short-chain fatty acid (SCFA) and lactate production were stimulated.
• Levels of anti-inflammatory cytokines increased, while pro-inflammatory cytokines and chemokines decreased.
• Epithelial tight-junction integrity improved, and wound healing accelerated with probiotic treatment.

Conclusions:

• The 4-strain probiotic suspension demonstrated a positive impact on gut microbiota composition and function.
• Probiotic intervention improved gut barrier integrity and modulated inflammatory responses.
• Dietary supplementation with this probiotic may serve as a beneficial adjunct therapy for Parkinson's disease, particularly if linked to gut dysbiosis.