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Updated: Oct 8, 2025
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Published on: July 29, 2018
Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC
Naoki Miyamoto1, Young-Hyun Go2, Larissa Miasiro Ciaramicoli3
1Center for Self-assembly and Complexity, Institute for Basic Science (IBS) Pohang 37673 Republic of Korea ytchang@postech.ac.kr.
Abstract:
CDy1 is a powerful tool to distingusih embryonic stem cells for reprogramming studies and regeneration medicine. However, the stem cell selectivity mechanism of CDy1 has not been fully understood. Here, we report ALDH2 and ABCB1 as the molecular targets of CDy1, elucidated by live-cell affinity-matrix and ABC transporter CRISPRa library screening. The two unique orthogonal mechanisms provide the potential of multi-demensional cellular distinction of specific cell types.
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