Related Experiment Video

Updated: Oct 8, 2025

Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells MBC-P and Biofilm Cells MBC-B
06:36

Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells MBC-P and Biofilm Cells MBC-B

Published on: January 2, 2014

20.2K

Efficacy of Common Antiseptic Solutions Against Clinically Relevant Planktonic Microorganisms

Jeffrey A O'Donnell, Mark Wu, Niall H Cochrane

    Orthopedics
    |January 3, 2022
    PubMed

    Insights

    Bactisure demonstrated superior efficacy in reducing planktonic microorganisms, while povidone-iodine and Irrisept showed variable results. Further research is needed to confirm their impact on preventing prosthetic joint infections.

    Area of Science:

    • Orthopedics
    • Infectious Diseases
    • Biomedical Engineering

    Background:

    • Prosthetic joint infections (PJIs) are severe complications following joint replacement surgery.
    • Limited evidence exists on the effectiveness of antiseptic solutions for reducing microbial burden.
    • Intraoperative irrigation is a key strategy for PJI prevention.

    Purpose of the Study:

    • To evaluate the in vitro efficacy of various antiseptic solutions against clinically relevant planktonic microorganisms.
    • To compare the antimicrobial activity of povidone-iodine, Irrisept, and Bactisure.

    Main Methods:

    • An in vitro experimental design was employed.
    • The study tested the efficacy of antiseptic solutions against planktonic microorganisms.
    • Microorganism reduction percentages were quantified for each solution.

    Main Results:

    • Bactisure achieved >99.9% reduction for all tested microorganisms.
    • Povidone-iodine showed >99.9% reduction, except for methicillin-resistant Staphylococcus aureus (60.44%).
    • Irrisept demonstrated >99.9% reduction, except for Staphylococcus epidermidis (98.31%) and Enterococcus faecalis (48.61%).

    Conclusions:

    • Significant differences in antiseptic efficacy against planktonic microorganisms were observed.
    • Bactisure exhibited the most comprehensive antimicrobial activity in vitro.
    • Clinical outcome data are required to determine the in vivo effectiveness of these irrigants in preventing PJIs.

    More Related Videos

    Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods11:47

    Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

    Published on: December 9, 2022

    1.8K
    Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries08:20

    Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries

    Published on: March 31, 2021

    6.5K

    Related Concept Videos

    Antimicrobial Effectiveness01:28

    Antimicrobial Effectiveness

    The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
    293
    Hand hygiene01:23

    Hand hygiene

    Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
    Hand washing...
    4.4K
    Chemical Agents for Microbial Control01:27

    Chemical Agents for Microbial Control

    Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
    304
    Surface Membrane Barriers01:18

    Surface Membrane Barriers

    The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
    The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
    1.7K
    Biological Methods for Microbial Control01:28

    Biological Methods for Microbial Control

    Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
    305
    Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

    Physical Methods for Controlling Microbial Growth: Radiation and Filtration

    Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
    344