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The influence of oxazolone on the recirculation capacity of B and T cells
Stimulation of lymph nodes with the contact allergen oxazolone had a profound effect on the recirculation capacity of IgD+ B cells and to a lesser extent of T cells when tested in an allotypic transfer system. The effect was most prominent on day 4 after stimulation and was not found with any other antigen that was tested. The impaired recirculation was correlated with a reduction of the adherence capacity of this cell population to HEV in vitro. However, the cells expressed normal levels of the MEL-14 antigen on their surface, as determined by dual-fluorescence FACS analysis. Similar effects were observed when lymphocytes were haptenized with the allergen for a short period in vitro. The treatment had no effect on the viability of the cell population nor on its capacity to react with lipopolysaccharide (LPS) and concanavalin A (Con A). When the cells were cultured for 24 h in the absence of oxazolone after either in vivo or in vitro treatment, a normal recirculation capacity was observed, indicating that the effects were reversible. The possible implications of this finding are discussed.
Stimulation of lymph nodes with the contact allergen oxazolone had a profound effect on the recirculation capacity of IgD+ B cells and to a lesser extent of T cells when tested in an allotypic transfer system. The effect was most prominent on day 4 after stimulation and was not found with any other antigen that was tested. The impaired recirculation was correlated with a reduction of the adherence capacity of this cell population to HEV in vitro. However, the cells expressed normal levels of the MEL-14 antigen on their surface, as determined by dual-fluorescence FACS analysis. Similar effects were observed when lymphocytes were haptenized with the allergen for a short period in vitro. The treatment had no effect on the viability of the cell population nor on its capacity to react with lipopolysaccharide (LPS) and concanavalin A (Con A). When the cells were cultured for 24 h in the absence of oxazolone after either in vivo or in vitro treatment, a normal recirculation capacity was observed, indicating that the effects were reversible. The possible implications of this finding are discussed.