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Updated: Oct 8, 2025
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Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models
Published on: August 9, 2012
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Rational development and evaluation of novel formulations for urinary health
Francesco De Seta1, Zoe Johnson2, Guglielmo Stabile3
1Department of Medical Sciences, University of Trieste, Institute for Maternal and Child Health- IRCCS, Burlo Garofolo, Trieste, Italy.
Summary
This study shows DAPAD, a D-mannose and prebiotic blend, inhibits urinary tract infection (UTI) bacteria in vitro. Further clinical evaluation may support DAPAD as a UTI treatment or preventative measure.
Area of Science:
- Urology
- Microbiology
- Pharmacology
Background:
- Urinary tract infections (UTIs) are common in women, driving interest in non-antibiotic treatments.
- DAPAD is a novel multi-functional composition explored for UTI-related biological activities.
Purpose of the Study:
- To provide preliminary data on the efficacy of DAPAD against common UTI pathogens.
- To investigate the biological activities of DAPAD components relevant to UTI prevention and treatment.
Main Methods:
- In vitro studies were conducted using four bacterial strains relevant to UTIs: E. coli, Proteus mirabilis, Streptococcus agalactiae, and Enterococcus faecalis.
- The formulation DAPAD included D-mannose, citric acid, prebiotics, and extracts of dandelion and astragalus.
Main Results:
- DAPAD demonstrated significant inhibition of all tested UTI bacteria at full and half strengths.
- D-mannose showed agglutination of E. coli and affected bacterial membrane integrity.
- Astragalus extract reduced cytokine elaboration by bladder epithelial cells, though dandelion and astragalus extracts did not inhibit bacterial growth.
Conclusions:
- DAPAD exhibits multi-functional properties relevant to UTI management.
- Further clinical studies are warranted to validate DAPAD's potential as a UTI treatment or preventative therapy.
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