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Expression pattern of Ptch2 in mouse embryonic maxillofacial development.

Xiaotong Wang1, Zhihong Ma2, Yan Wu3

  • 1Laboratory of Molecular Signaling and Stem Cells Therapy, Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Tiantan Xili No. 4, Beijing 100050, China.

Acta Histochemica
|January 3, 2022
PubMed
Summary

Patched (Ptch)2 is crucial for facial development. This study details its expression patterns in mouse embryos, revealing its role in craniofacial structures and suggesting its importance in normal embryonic facial development.

Keywords:
Facial developmentFollicles of vibrissaeMeckel’s cartilagePtch2Tooth germ

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Embryogenesis relies on complex signaling pathways, including Hedgehog (Hh) signaling.
  • Patched (Ptch)2, a homolog of Ptch1, is increasingly recognized for its role in Hh signaling feedback inhibition.
  • The specific function of Ptch2 in facial development is not well understood.

Purpose of the Study:

  • To investigate the expression pattern of Patched (Ptch)2 during craniofacial development in murine embryos.
  • To elucidate the potential role of Ptch2 in the development of facial structures.

Main Methods:

  • Whole-mount and section in situ hybridization (ISH) for mRNA expression.
  • Immunohistochemistry (IHC) for protein localization.
  • Quantitative real-time PCR (qPCR) for gene expression analysis.

Main Results:

  • Ptch2 mRNA and protein expression showed dynamic increases during mouse embryonic facial development (days 11-14.5).
  • Distinct Ptch2 expression was observed in key facial structures: tooth germ, Meckel's cartilage, and vibrissae follicles.
  • Expression patterns suggest a role in the development of these specific craniofacial components.

Conclusions:

  • Ptch2 exhibits dynamic expression during critical stages of murine craniofacial development.
  • Its localized expression in facial structures indicates a significant role in their formation.
  • These findings suggest Ptch2 is a critical factor in vertebrate facial development.