Nanozyme-strip based on MnO2 nanosheets as a catalytic label for multi-scale detection of aflatoxin B1 with an ultrabroad working range
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Updated: Oct 8, 2025
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
Xinfa Cai1, Meijuan Liang1, Fei Ma1
1Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, PR China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture and Rural Affairs, Wuhan 430062, PR China.
Abstract:
Lateral flow immunoassay (LFIA) is the most effective real-time detection method for aflatoxin B1 (AFB1). Here, we constructed a nanozyme-strip based on MnO2 nanosheets (MnO2 NSs) as a catalytic label for detection of AFB1. By taking advantage of the MnO2-TMB catalytic amplified system, the new test achieves rapid detection with high sensitivity and ultrawide range. The limit of detection of the assay was 15 pg mL-1, which was over 100-fold lower than the maximum limit set by the European Union (EU) of AFB1 in foods. In addition, the strip test could offer 7 dynamic detection ranges, spanning 4 orders of magnitude, which could cater to the varieties of limits on AFB1 residues in foods and feeds set by different countries. The estimated recoveries were in the range of 85.67%-106.38% with coefficients of variations (CVs) less than 9.68%. Overall, the developed approach is a rapid, reliable, sensitive and widely available tool for on-site detection of AFB1.
