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Related Concept Videos

In Vitro Drug Dissolution: Compendial Testing Models II01:09

In Vitro Drug Dissolution: Compendial Testing Models II

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Various dissolution methods are utilized to assess a drug’s dissolution rate, including the flow-through cell, paddle-over-disk, cylinder, and reciprocating disk methods.The flow-through cell apparatus (USP (United States Pharmacopeia) method 4) comprises a reservoir for the dissolution medium and a pump that propels the medium through the cell containing the test sample. This method is crucial for assessing modified-release dosage forms with minimally soluble active ingredients,...
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New in vitro SPF Evaluation Method for Hydrophilic Sunscreen Samples.

Kurumi Ikawa1, Amiko Aizawa1, Taisuke Banno1

  • 1Department of Applied Chemistry, Faculty of Science & Technology, Keio University.

Journal of Oleo Science
|January 4, 2022
PubMed
Summary

A novel hydroxyalkyl cellulose-coated plate offers a reliable in vitro method for evaluating sunscreen's sun protection factor (SPF), particularly for hydrophilic formulations. This new substrate ensures consistent wetting and accurate SPF measurements, outperforming standard plates.

Keywords:
in vitro SPF evaluation methodPMMA platehydroxyalkyl cellulose coated platesunscreensuper-hydrophilic plate

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Area of Science:

  • Cosmetic Science
  • Dermatology
  • Analytical Chemistry

Background:

  • Standard in vitro sun protection factor (SPF) evaluation methods face challenges with hydrophilic sunscreen formulations due to poor substrate wetting.
  • Existing methods using polymethyl methacrylate (PMMA) plates are unsuitable for samples that do not wet the hydrophobic surface.
  • A need exists for a more versatile and reliable in vitro SPF testing substrate for diverse cosmetic products.

Purpose of the Study:

  • To develop and validate a new in vitro method for SPF evaluation using a novel hydroxyalkyl cellulose-coated plate.
  • To assess the performance of this new substrate for hydrophilic sunscreen samples compared to existing plates.
  • To establish a simplified and robust in vitro SPF evaluation protocol.

Main Methods:

  • Preparation of a hydroxyalkyl cellulose-coated plate via corona-discharge treatment and cellulose coating on a quartz substrate.
  • Application of six hydrophilic sunscreen samples with known in vivo SPF values onto the coated plate, a super-hydrophilic quartz plate, and a PMMA plate.
  • Measurement of wet film thickness and UV transmission using an SPF analyzer to calculate in vitro SPF values.

Main Results:

  • The hydroxyalkyl cellulose-coated plate successfully accommodated all hydrophilic samples, unlike the PMMA plate which failed for two samples.
  • In vitro SPF values obtained using the hydroxyalkyl cellulose-coated plate and super-hydrophilic quartz plate showed minimal differences.
  • The coated plate demonstrated long-term hydrophilicity stability (over six months) compared to the super-hydrophilic quartz plate (approx. five days).

Conclusions:

  • The hydroxyalkyl cellulose-coated plate provides a stable and effective substrate for in vitro SPF evaluation of hydrophilic sunscreens.
  • This method offers a viable alternative to standard techniques, improving accuracy and applicability for challenging formulations.
  • A simplified evaluation method based on this substrate was validated through a multi-laboratory ring test.