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Published on: July 23, 2012
Analysis of Micro-RNA-144 Expression Profile in Patients with Multiple Sclerosis in Comparison with Healthy
Fatemeh Roshani1, Hossien Delavar Kasmaee2, Kowsar Falahati3
1Department of Genetics, Nourdanesh Institute of higher Education, Myme, Esfahan, Iran.
Background:
Etiology of multiple sclerosis is non-clarified. It seems that environmental factors impact epigenetic in this disease. Micro-RNAs (MIR) as epigenetic factors are one of the most important factors in non-genetically neurodegenerative diseases. It has been found MIR-144 plays a main role in the regulation of many processes in the central nervous system. Here, we aimed to investigation of MIR-144 expression alteration in Multiple sclerosis (MS) patients.
Methods:
In this study 32 healthy and 32 MS patient's blood sample were analyzed by quantitative Real-Time PCR method and obtained data analyzed by REST 2009 software.
Results:
Analysis of Real-Time PCR data revealed that miR-144 Increase significantly in MS patients compared to healthy controls.
Conclusion:
The increase of MIR-144 expression in MS patients is obvious. MIR-144 can be used as a biomarker of MS and help to early diagnosis and treatment of this disease.
