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Nano-LC: An updated review.
1LC-MS Biologics, QSquared Solutions, Ithaca, New York, USA.
Biomedical Chromatography : BMC
|January 4, 2022
Summary
Nano-flow liquid chromatography (LC) offers enhanced sensitivity and selectivity for bioanalysis. Advances in instrumentation and multidimensional separations are crucial for meeting the high-throughput demands of modern biopharmaceutical development.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Chromatography
Background:
- Low-flow chromatography has historical innovations but limited bioanalytical implementation.
- Recent advancements in pump technology, microfluidics, and nano-electrospray ionization mass spectrometry (MS) have accelerated innovation.
- The increasing complexity of biopharmaceuticals necessitates highly sensitive and selective analytical platforms.
Purpose of the Study:
- To review instrumentation for nano-flow LC.
- To discuss column types and multidimensional separation strategies for nano-LC.
- To update current applications and highlight future potential in bioanalysis.
Main Methods:
- Review of nano-flow liquid chromatography instrumentation.
- Analysis of column technologies for nano-LC.
- Exploration of multidimensional separation techniques.
- Discussion of nano-electrospray ionization MS for detection.
Main Results:
- Nano-LC instrumentation, columns, and multidimensional strategies are evolving.
- Current applications in proteomics and metabolomics are established.
- Nano-LC coupled with high-resolution MS and nano-ESI provides high selectivity and sensitivity.
Conclusions:
- Nano-flow LC is becoming an accessible, robust, and user-friendly platform.
- It is essential for the targeted quantitation of complex biotherapeutics in regulated environments.
- Continued evolution supports high-throughput bioanalysis and biopharmaceutical development.
