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X-ray Crystallography

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

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Related Experiment Video

Updated: Oct 8, 2025

Fixed Target Serial Data Collection at Diamond Light Source

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

Best practices for time-resolved serial synchrotron crystallography.

Eike C Schulz¹, Briony A Yorke², Arwen R Pearson¹

¹Institute for Nanostructure and Solid State Physics, Universität Hamburg, HARBOR, Luruper Chaussee 149, 22761 Hamburg, Germany.

Acta Crystallographica. Section D, Structural Biology

|January 4, 2022

Summary

This summary is machine-generated.

Time-resolved crystallography experiments are becoming more accessible due to technological advancements. Careful planning and execution are crucial for successful time-resolved structural studies, especially at synchrotron sources.

Keywords:

SSX mechanistic structural biology serial crystallography structural enzymology time-resolved crystallography

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Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

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Last Updated: Oct 8, 2025

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Published on: February 26, 2021

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Area of Science:

Structural Biology
Biophysics
Crystallography

Background:

Advancements in X-ray sources, instrumentation, and data analysis have simplified time-resolved crystallographic experiments.
These experiments are becoming more accessible to a broader user base beyond expert groups.
Time-resolved crystallography offers unique insights into dynamic biological processes.

Purpose of the Study:

To outline key factors for planning and executing successful time-resolved structural studies.
To highlight considerations specific to synchrotron-based experiments.
To guide researchers in optimizing time-resolved crystallographic workflows.

Main Methods:

Focus on planning and experimental design for time-resolved crystallography.
Consideration of synchrotron radiation sources and their capabilities.
Emphasis on data collection strategies and potential challenges.

Main Results:

Time-resolved crystallographic experiments require meticulous planning beyond simple data collection.
Accessibility of these techniques is increasing.
Synchrotron-based experiments present specific considerations for successful outcomes.

Conclusions:

Successful time-resolved structural studies depend on careful experimental planning and execution.
Researchers should be aware of potential pitfalls in time-resolved crystallography.
The accessibility of time-resolved crystallography is growing, necessitating best practices for its application.