Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:26

Membrane Fluidity

12.8K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
12.8K
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

3.3K
Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
3.3K
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

8.4K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
8.4K
Membrane Domains01:18

Membrane Domains

6.1K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
6.1K
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

3.5K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.5K
Lipids as Anchors01:32

Lipids as Anchors

6.3K
In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
6.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cadherin regulation of endoplasmic reticulum-plasma membrane contact sites.

Cell reports·2026
Same author

Large-scale endoplasmic reticulum membrane solidification spatially organises proteins under thermal or metabolic stress.

bioRxiv : the preprint server for biology·2026
Same author

Nanoscale Spatial Organization of ARC High- and Low-Order Assemblies at Excitatory Synapses.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Inherent Lipid Composition Abnormalities in Astrocytes Associated with Late-Onset Alzheimer's Disease (LOAD).

Cells·2026
Same author

Sex-specific KDM6A-HNF4A-CREBH network controls lipoprotein cholesterol metabolism and atherosclerosis via epigenetic reprograming of hepatocytes.

Nature communications·2026
Same author

Studying Macromolecular Composition in Cell-Cell Interfaces Using 3D Membrane Reconstitution Systems.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Related Experiment Video

Updated: Oct 8, 2025

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs
09:45

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs

Published on: February 5, 2022

3.7K

Lipid-Protein Interactions in Plasma Membrane Organization and Function.

Taras Sych1, Kandice R Levental2, Erdinc Sezgin1,3

  • 1Science for Life Laboratory, Department of Women's and Children's Health, Karolinska Institutet, Solna, Sweden; email: taras.sych@ki.se, erdinc.sezgin@ki.se.

Annual Review of Biophysics
|January 4, 2022
PubMed
Summary

Cellular lipid-protein interactions are crucial for biological processes. New technologies reveal how these interactions and membrane lipid behavior influence protein function.

Keywords:
collective membrane propertiescryo-EMlipid-binding motifsmembrane biophysicsmolecular dynamicssuper-resolution imaging

More Related Videos

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

2.6K
PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
10:58

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

Published on: July 27, 2017

9.6K

Related Experiment Videos

Last Updated: Oct 8, 2025

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs
09:45

Construction of Model Lipid Membranes Incorporating G-protein Coupled Receptors GPCRs

Published on: February 5, 2022

3.7K
Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

2.6K
PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
10:58

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

Published on: July 27, 2017

9.6K

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Biophysics

Background:

  • Lipid-protein interactions are fundamental to cellular functions, including metabolism, signaling, and transport.
  • These interactions are particularly critical at the plasma membrane, influencing membrane organization and protein function.
  • Specific lipid-binding motifs on proteins and the collective behavior of membrane lipids dynamically regulate protein activity.

Purpose of the Study:

  • To review recent literature on lipid-protein interactions.
  • To highlight emerging technologies for studying these interactions.
  • To provide insights into how lipid-protein interactions and membrane dynamics affect protein function.

Main Methods:

  • Review of recent scientific literature.
  • Focus on analytical, biochemical, and computational technologies.
  • Analysis of studies investigating specific lipid-protein binding and collective lipid behavior.

Main Results:

  • Lipid-protein interactions modulate protein conformation and function through specific binding motifs.
  • The collective, dynamic behavior of membrane lipids also significantly regulates protein activity.
  • Advancements in technology are providing unprecedented insights into these complex relationships.

Conclusions:

  • Emerging technologies are revolutionizing the study of lipid-protein interactions.
  • Understanding these interactions is key to deciphering various cellular processes.
  • Future research will further elucidate the intricate roles of lipids in protein function and cellular dynamics.